372-pAd5-CMV-PKM2
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Construction of pAd5-CMV-PKM2 372
[edit]Purpose: construction of pAd5-CMV-PKM2 (372).
Date: 2011-8-31 to 2011-9-
Notebook:
Page:
Clone ID:
Experimenters: Paul
Results
[edit]Digestion
[edit]Date: 2011-8-31
Digest pET3a-Muts (clone #352) with Nde1+ Sph1
Digest pDB.His.MBP (clone # 360 ) with Nde1+ Sph1
cut out insert MBP and vector pET3a-Muts , ligate and transform Top10
--Paul.xu 13:02, 31 August 2011 (EST)
digest PCR product and vector in one tube with BamH1 + Xho1, run gel and purify, ligate, transform stbl3.
--Paul.xu 17:34, 31 August 2011 (EST)
Colony Screening
[edit]Date: 2011-9-1
Result intepretation/Summary
[edit]Conclusion
[edit]Restriction digestion
Shows