3C digestion
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Use 1 mg 3C protease to digest 100 mg fusion protein.
Buffer
50 mM Tris-HCl, 150 mM NaCl, 1 mM dithiothreitol (DTT), pH 7.0 ~8.0.
Use 1 mg 3C protease to digest 100 mg fusion protein.
Buffer
50 mM Tris-HCl, 150 mM NaCl, 1 mM dithiothreitol (DTT), pH 7.0 ~8.0.