3 A Tailing

Prepare the following reaction mixture
a) Purified DNA in water 32 μl
b) 10X buffer 5 μl
c) 1 mM dATP 10 μl
d) Klenow Exo- 3 μl
total volume 50 μl

Incubate in a heat block at 37°C for 30 minutes.

Protocol 2

Purified DNA 44µl

5 µl 10X Taq buffer

1 μL 10 mM dATP

0.5 µL Taq polymerase.

incubate at 72°C for 30 minutes