458-pLenti-CymR
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Cloning of 458-pLenti-CymR
[edit]Purpose: Subloning of 458-pLenti-CymR from 457 pET28b-CymR to 152 pLenti-Plus
Date: 2011-12-16---12-23
Notebook:
Page:
Clone ID:458
Experimenters: yingying
Results
[edit]Subcloning
[edit]Date: 2011-12-22
| band |
band 1 |
band2 |
| 457 pET28b-CymR |
152 pLenti-Plus | |
| digest enzyme |
BamH1 + Xho1 |
BamH1 + Xho1 |
combine insert above together
Colony Screening
[edit]Date: 2011-12-23
Make miniprep and digest with BamH1 + Xho1
8 clonies
Result intepretation/Summary
[edit]sequence result: 414-6 right
Conclusion
[edit]414-6 right