461-pAAV-CMV-Cre-mKate2
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Cloning of pAAV-CMV-Cre-mKate2
Purpose: to clone gene synthesis to 284 pAAV-CMV-mkate2
Date: 2011-11-29
Notebook:Lu yingying
Page:
Clone ID:461
Experimenters: lu yingying
Results
PCR the same to 454 PCR
Cre-EcoR1-F
aacta gaattc gccacc ATGGGCCATCACCATCACCATCACG
Cre-BglII-R
(CGCCTGCTGGAAGATGGCGAT ggA GAT CT atcag )
ctgatAGATCTccATCGCCATCTTCCAGCAGGCG
PCR using pTriEx-HTNC 298 as template for PCR,---> 1158 bp
digest result
band 3 show PCR product digest with EcoR1 + BglII
band 4 show vector 284 pAAV-CMV-mkate2 with EcoR1 + BamH1
digest detect:EcoR1+Xho1