470-pAAV-CMV-RFP
Cloning of 470-pAAV-CMV-RFP
Purpose: to subclone from pET28b-TagRFP 114 to pAAV.CMV-MCS 254
Date: 2011-10-20----
Notebook:Lu yingying
Page:
Clone ID:470
Experimenters: lu yingying
Results
band show digest result: BamH1 + Xho1 NEB enzyme for 2 hours
band 1 show 254-pAAV.CMV-MCS vector
band 2 show 114 pET28b-TagRFP insert
ligation RT for 3 hours
Restart
Date: 2011-10-21----
Notebook:Lu yingying
Page:
Clone ID:470
Experimenters: lu yingying
Results
band show digest result: BamH1 + Xho1 NEB enzyme for 2 hours
band 1,2 show 254-pAAV.CMV-MCS vector
band 3,4 show 114 pET28b-TagRFP insert
Date: 2011-10-25----
Notebook:Lu yingying
Page:
Clone ID:470
Experimenters: lu yingying
Results
band 1 shows 114 pET28b-TagRFP insert
band 4 show 254-pAAV.CMV-MCS vector
ligation 2 hours RT
470 1-4 bands show digest detect:BamH1 + Xho1