472-pAd5.CMV-MCS2

Cloning of pAd5.CMV-MCS2 (472)
Purpose: to clone pAd5.CMV-MCS2 
Date: 2011-12-20 to 2012-1-5
Notebook: Xiaojing Wang
Page: 004148---004148
Clone ID: 472
Experimenters: Xiaojing Wang
Results

1 annealed oligos:  

pAD5-MCS-T4 E7 CGGATCCtctagaGAATTCACCGGTAAGCTTCTCGAGCCTAGGGC
pAD5-MCS-B4 E8 GGCCGCctcgagCACGTGAAGCTTtctagaggatccACCGGTgaattcGGTAC

2 digest pAd5-CMV-EGFP(304) with Kpn1 + Not1, cut out vector. Control is 304 plasmid.

Ligation, Transformation

Select 10 clones to mini-prep
472 plasmids are digested by Nde1+BamH1