475-PET28b-pfu

cloning of 47-PET28b-pfu

Purpose: to clone PET28b-pfu by gene synthesis to PET28b

Date: 2011-11-29----

Notebook:Lu yingying

Page:

Clone ID:475

Experimenters: lu yingying
Results

PCR

template: 381pSpeedET-Pfu

primer: Pfu-F2 + Pfu-KpnI-R

digest: Nde1+ Kpn1

band 1 digested PCR insert

band 2 digested pET28b

digest detect: Nde1+ Kpn1

Date: 2011-12-12

Notebook:Lu yingying

Page:

Clone ID:477

Experimenters: lu yingying
Results

digest: Nde1+ Kpn1

band 1 digested PCR insert

band 2 digested pET28b

digest detect: Nde1+ Kpn1

Cloning of 475-PET28b-pfu

Purpose: Cloning of 475-PET28b-pfu by PCR

Date: 2011-12-20

Notebook: 004061--

Page:

Clone ID:475

Experimenters: yingying

Results

	band1	band2
	PCR product	PET28b
digest	Nde1+ Kpn1	Nde1+ Kpn1

Subcloning

Date: 

Colony Screening

Date:2011-12-23

Make miniprep and digest with Nde1+ Kpn1

 
Result intepretation/Summary