477-PET30b-MMLV-N

Cloning of 477-PET30b-MMLV-N

Purpose: to clone PET30b-MMLV-N by PCR

Date: 2011-12-5----

Notebook:Lu yingying

Page:

Clone ID:477

Experimenters: lu yingying
Results

Gene synthesis of fragment 1 :MMLV-1 to MMLV-30

digest with Nde1 + Nco1, clone into Nde1 + Nco1 digested pET30b

--Yingying.lu 15:14, 9 December 2011 (EST)

Date: 2011-12-12

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Notebook:Lu yingying

Page:

Clone ID:477

Experimenters: lu yingying
Results

digest with Nde1 + Nco1, clone into Nde1 + Nco1 digested pET30b

Cloning of 477-PET30b-MMLV-N

Purpose: Cloning of 477-PET30b-MMLV-N

Date: 2011-12-20---

Notebook:

Page:

Clone ID: 477

Experimenters: yingying

Results

	band 3	band 4
	PCR product	PET30b
digest	Nde1 + Nco1	Nde1 + Nco1

Subcloning

Date: 

Colony Screening

Date: 2011-12-23

Make miniprep and digest with Nde1 + Nco1

clone 2,3,5,6,7 may right, need to sequence