478-pAd5.CMV-CuO-MCS
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Cloning of pAd5.CMV-CuO-MCS
Purpose: to clone pAd5.CMV-CuO-MCS form 448 pLex-CMV-CuO to pAd5-CMV-MCS 280
Date: 2011-12-13---
Notebook:Lu yingying
Page:
Clone ID:478
Experimenters: lu yingying
Results
pAd5-CMV-MCS 280 with Nde1 + BamH1, Digest 448 pLex-CMV-CuO with Nde1 + BamH1
--Yingying.lu 12:36, 13 December 2011 (EST)
Cloning of 478-pAd5.CMV-CuO-MCS
[edit]Purpose: Cloning of 448 pLex-CMV-CuO to pAd5-CMV-MCS 280
Date: 2011-12-27
Notebook:
Page:
Clone ID: 478
Experimenters: yingying
Results
[edit]Subcloning
[edit]Date: 2011-12-27
| band 1 |
band 2 | |
| 448 pLex-CMV-CuO |
pAd5-CMV-MCS 280 | |
| digest enzyme |
Nde1 + BamH1 | |
Colony Screening
[edit]Date: 2011-12-29
Make miniprep and digest with Nde1 + BamH1
clone 1,2,3,5,6,7,8 may be right
Result intepretation/Summary
[edit]- Sequencing Result:
Conclusion
[edit]
[edit]
--Yingying.lu 15:57, 29 December 2011 (EST)