490-pPinka-HC2-Ent
Jump to navigation
Jump to search
Cloning of 490-pPinka-HC2-Ent
Purpose: to 490-pPinka-HC2-Ent by PCR
Date: 2011-12-12----
Notebook:Lu yingying
Page:
Clone ID:490
Experimenters: lu yingying
Results
PCR using these two primers
Enterokinase1 ATACGCCATGGATTGTCGGAGGAAGTGACTCC
Xho1-6XHis-EcoR1-R tcgatGAATTCTCAGTGGTGGTGGTGGTGGTGCTCGAG
and pET30b-Ent 345 as template
--Yingying.lu 15:28, 12 December 2011 (EST)