512-pET8XHis-Taq
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Cloning of 512-pET8XHis-Taq
[edit]Purpose: Subcloning of 512-pET8XHis-Taq from pET28b-Taq 176 to pET8XHis 286
Date: 2011-12-15----
Notebook:
Page:
Clone ID:512
Experimenters: yingying
Results
[edit]Subcloning
[edit]Date: 2011-12-15
| band 1 |
band2 |
band 3 | |
| pET28b-Taq 176 |
pET28b-Taq 176 |
pET8XHis 286 | |
| digest |
Nco1 + Sal1 | ||
wild type
wild type :Wt-Taq-XuYan, 462
Nco1 + Sal1
Colony Screening
[edit]Date:12-30-2011
Make miniprep and digest with Nco1 + Sal1
Result intepretation/Summary
[edit]Conclusion
[edit]
[edit]
--Yingying.lu 09:23, 3 January 2012 (EST)