519-pAD5.CAG-mMKOS

Cloning ofpAD5.CAG-mMKOS (519)
Purpose: to clone pAD5.CAG-mMKOS by subclone
Date: 2011-12-30 to 2012-1-5
Notebook: Xiaojing Wang
Page: 004147---004147
Clone ID: 519
Experimenters: Xiaojing Wang
Results 

Cut out KOSM from pLVX2MKOSiE 314 using Nde1 + BamH1, the insert is 6.5 kb.
Subclone into Nde1 + BamH1 digested pAd5-CMV-MCS 280.

ligation, transformation

select 5 clones to mini-prep

519 plasmids are digested by Nde1+BamH1,519-1,-3,-4 plasmids may be correct. 

519-1,-3,-4 plasmids are digested by xho1.