521-pCDNA3.1-E1B-19K

Cloning of pCDNA3.1-E1B-19K (521)

Purpose: to clone pCDNA3.1-E1B-19K by Gene Synthesis

Date: 2012-1-18 to 2012-1-23

Notebook: Xiaojing Wang

Page: 004163---004164

Clone ID: 521

Experimenters: Xiaojing Wang

Results

digest:

digest PCR product and vector with EcoR1 +Xho1,run gel

the left band shows 521 insert, the right picture shows 335 vector (pCDNA3.1, 335)

gel purify, ligation, transformation

Colony Screening

Select 5 clones to mini-prep, digestd by EcoR1 and Xho1. 521-1,-3,-4,-5 may be correct. Control is 521-1.

Select 521-1 to sequence.