522-pCDNA3.1-Bcl-X

Cloning of pCDNA3.1-Bcl-X (522)

Purpose: to clone pCDNA3.1-Bcl-X by Gene Synthesis

Date: 2012-1-18 to 2012-1-23

Notebook: Xiaojing Wang

Page: 004163---004164

Clone ID: 522

Experimenters: Xiaojing Wang

Results

digest:

digest PCR product and vector with Nhe1 +Xho1,run gel

the left band shows 522 insert, the right band shows 335 vector (pCDNA3.1, 335)

gel purify, ligation, transformation

Colony Screening

Select 5 clones to mini-prep, digestd by Nhe1 and Xho1. 522-2, -3, -5 may be correct. Control is 522-1.

Select 522-3 to sequence.