524-pCDNA3.1-E1A

Cloning of pCDNA3.1-E1A (524)

Purpose: to clone pCDNA3.1-E1A by Gene Synthesis

Date: 2012-1-18 to 2012-1-23

Notebook: Xiaojing Wang

Page: 004163---004164

Clone ID: 524

Experimenters: Xiaojing Wang

Results

digest:

digest PCR product and vector with EcoR1 +Xho1,run gel

the left band shows 524 insert, the right picture shows 335 vector (pCDNA3.1, 335)

gel purify, ligation, transformation

Colony Screening

Select 5 clones to mini-prep, digestd by EcoR1 and Xho1. 524-1,-3,-4,-5 may be correct.

Select 524-1 to sequence.