533-pLVX-Cre-IRES-EGFP
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Cloning of 533-pLVX-Cre-IRES-EGFP
[edit]Purpose: Cloning of 533-pLVX-Cre-IRES-EGFP by subclone from 414 pAd5-CMV-Cre to 528 pLVX-IRES-EGFP
Date: 2012-1-13--
Notebook:
Page:
Clone ID:533
Experimenters: yingying
Results
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Subcloning
[edit]Date: 2012-1-13
| band 1 |
band 2 | |
| 414 pAd5-CMV-Cre |
528 pLVX-IRES-EGFP | |
| digestion enzymes |
EcoR1+Xho1 | |
Colony Screening
[edit]Date: 2012-1-17
Make miniprep and digest with EcoR1+Xho1
clone 1,2,3,4 may right
transfection result: green fluorescense positive
Result intepretation/Summary
[edit]Conclusion
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