543-pTracer-Pani2A
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Cloning of 543-pTracer-Pani2A
[edit]Purpose: Cloning of 543-pTracer-Pani2A by PCR
Date: 2012-1-26--
Notebook:003719
Page:
Clone ID:543
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-1-26
PCR using Pani2A 540 as template
Primer-F Pani2A-F AGGGAGACCCAAGCTGGCTAGC gcc acc ATGGACGCCA TGCTGCGAGG
Primer-R Pani2A-R (CAACCGAGGCGAGTGCTGA GCGGCCGC TCGAGTCTAGAGGGCCCGT) ACGGGCCCTCTAGACTCGAGCGGCCGCTCAGCACTCGCCTCGGTTG
| band 2 | |
| PCR product | |
| digest enzymes |
Nhe1 + Not 1 |
| band | |
| pTracer.CMV/Bsd 538 | |
| digest enzymes |
Nhe1 + Not 1 |
Colony Screening
[edit]Date: 2012-1-30
Make miniprep and digest with Nhe1 + Not1
clone 1,2,3,4,5 may be right, need to sequence
Result intepretation/Summary
[edit]Conclusion
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