544-pTracer-SPbacCD
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Cloning of 544-pTracer-SPbacCD
[edit]Purpose: Cloning of 544-pTracer-SPbacCD by PCR
Date: 2012-1-26--
Notebook:003719
Page:
Clone ID:544
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-1-26
PCR template is SPbacCD 541
Primer-F SPbacCD-F AGGGAGACCCAAGCTGGCTAGC gcc acc ATG GAC GCC ATG CTG CGA GG
Primer-R SPbacCD-R (GCTATTGACTACAAAAGGTGA GCGGCCGC TCGAGTCTAGAGGGCCCGT) ACGGGCCCTCTAGACTCGAGCGGCCGCTCACCTTTTGTAGTCAATAGC
| band 3 | |
| PCR product | |
| digest enzymes |
Nhe1 + Not1 |
| band | |
| pTracer.CMV/Bsd 538 | |
| digest enzymes |
Nhe1 + Not1 |
Colony Screening
[edit]Date: 2012-1-30
Make miniprep and digest with Nhe1 + Not1
clone 1,2,3,4,5 may be right, need to sequence
Result intepretation/Summary
[edit]Conclusion
[edit]