553 649 715 636 638 640 641 645 1504 2348 2196

Protein Expression

2014/10/06
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw	express
1	pET28b-Xho1		553	15 ml, Tuner(DE3) pLysN-Plac-mXho1	0.4 mM IPTG  37oC 2h	Ni beads
2	pT7A3-Xho1		649	1 5 ml, Tuner(DE3) pLysN-Plac-mXho1	0.5 mM IPTG 37oC 2h	Ni beads
3	pT7A3E-wt-Xho1		715	1 5 ml, Tuner(DE3) pLysN-Plac-mXho1	0.5 mM IPTG 37oC 2h	Ni beads
4	PageRuler Plus Prestained protein ladder
5	pT7A3-XbaI		636	15 ml, Tuner(DE3) pLysN-ha-Plac-hia5	0.5 mM IPTG 37oC 2h	Ni beads
6	pT7A3-BglII		638	15 ml, Tuner(DE3) pLysN-ha-Plac-hia5	0.5 mM IPTG 37oC 2h	Ni beads
7	pT7A3-EcoRI		640	1 5 ml,	0.5 mM IPTG 37oC 2h	Ni beads
8	pT7A3-EcoRV		641	15 ml, Tuner(DE3) pLysN-ha-Plac-hia5	0.5 mM IPTG 37oC 2h	Ni beads
9	pT7A3-PstI		645	15 ml, Tuner(DE3) pLysN-ha-Plac-hia5	0.5 mM IPTG 37oC 2h	Ni beads
10	pT7A3-T4DNA-Pol-Exo		1504	15 ml, Nico21(DE3)ppLysN	0.5 mM IPTG 37oC 2h	Ni beads
11	pT7A3-MBP-HCMVUL98		2348	15 ml, Nico21(DE3)ppLysN	0.5 mM IPTG 37oC 2h	Ni beads
12	pT7A3E-PBCV-1-Lig		2196			Ni column