555-pET28b-Hind111
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Cloning of 555-pET28b-Hind111
[edit]Purpose: Cloning of 555-pET28b-Hind111
Date: 2013-6-3--2013-6-7
Notebook:0
Page:
Clone ID:555
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1: 1-1 to 1-14
pcr2:2-1 to 2-12
pcr:1-1 to 2-12
clone into pET28b Nde1+Xho1
Colony Screening
[edit]Date: 2013-6-7
Make miniprep and digest with: nde1+xho1
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]fix of Cloning of 555-pET28b-Hind111
[edit]Purpose: fix of Cloning of 555-pET28b-Hind111
Date: 2013-6-20---2013-6-24
Notebook:0
Page:
Clone ID:555
Experimenters: yingying
Results
[edit]cloning
[edit]
555-4 above20120607 as template
pcr1--primer:1-1 to 2-8
pcr2--primer:2-9 to 2-12
template:pcr1+pcr2
primer :1-1 and 2-12
lane1: clone into vector 1225pET28b-T7-Endo1
nde1+xho1
Colony Screening
[edit]Date: 2013-6-24
Make miniprep and digest with: nde1+xho1
Result intepretation/Summary
[edit]- <br>