623 625 637 1494 1499 1734 1735 1740 1747 1750

Protein Expression

2013/5/31
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw
1	pT7A3-8H-IL-4	IL-4	623	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTGRT o/n	Ni beads	9.13/17492.34
2	pT7A3-8H-IL-15	IL-15	625	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	5.13 / 18085.85
3	pT7A3-Nco1	Nco1	637	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.36 / 31590.37
4	pT7A3-UDG	UDG	1494	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	5.45 / 27174.73
5	pT7A30-RNaseInhibitor	Sus scrofa (wild pig) RNase Inhibitor	1499	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	4.76 / 49023.27
M	PageRuler Plus Prestained protein ladder
6	pT7A30-sso7d-KX	sso7d	1734	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	7.26 / 13790.44
7	pT7A30-Exo-Taq-sso7d	Exo-Taq-sso7d	1735	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.35 / 98021.85
8	pT7A3-8HZB2	8HZB2	1740	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	9.81 / 8950.02
9	pT7A30-iMMLV	MMLV	1747	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT o/n	Ni beads	9.10 / 74587.10
10	pT7A3-Tks	Tks DNA Polymerase	1750	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.95 / 92850.10