635 636 637 638 639 640 641 643 644 645 649

Protein Expression

Nico21(DE3)plysN 0.2 mM IPTG RT o/n Tuner(DE3)plysN 0.2 mM IPTG RT o/n Tuner(DE3)plysN 0.2 mM IPTG 15oC o/n

2013/8/15
SHANYI

Lane	Vector Name	Name	Clone ID	Host	Induction	Purification	pI/Mw	Expression
1	pT7A3-Not1	Not1	635	5 ml, Nico21(DE3)plysN	0.2 mM IPTGRT o/n	Ni beads	5.64/47kD
2	pT7A3-Xba1	Xba1	636	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.94/28.4kd
3	pT7A3-Nco1	Nco1	637	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.47/36kd
4	pT7A3-BglII	BglII	638	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.66/31kd
5	pT7A3-BamHI	BamHI	639	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.47/29.6kd	YES
6	pT7A3-EcoR1	EcoR1	640	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.96/36.5kd
7	pT7A3-EcoRV	EcoRV	641	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.62/32.7kd
8	PageRuler Plus protein ladder
9	pT7A3-Nhe1	Nhe1	643	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.06/42.7kd
10	pT7A3-SalI	SalI	644	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT o/n	Ni beads	6.01/31kd	YES
11	pT7A3-PstI	PstI	645	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	7.32/41kd
12	pT7A3-Xho1	Xho1	649	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.66/32.3kd	YES

2013/8/21
SHANYI

Lane	Vector Name	Name	Clone ID	Host	Induction	Purification	pI/Mw	Expression
1	pT7A3-Not1	Not1	635	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.64/47kD
2	pT7A3-Xba1	Xba1	636	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.94/28.4kd
3	pT7A3-Nco1	Nco1	637	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.47/36kd	yes
4	pT7A3-BglII	BglII	638	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.66/31kd
5	pT7A3-BamHI	BamHI	639	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.47/29.6kd	YES
6	pT7A3-EcoR1	EcoR1	640	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.96/36.5kd
7	pT7A3-EcoRV	EcoRV	641	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.62/32.7kd
8	PageRuler Plus protein ladder
9	pT7A3-Nhe1	Nhe1	643	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.06/42.7kd
10	pT7A3-SalI	SalI	644	5 ml, Tuner(DE3)plysN	0.2mM IPTG RT o/n	Ni beads	6.01/31kd
11	pT7A3-PstI	PstI	645	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	7.32/41kd	YES
12	pT7A3-Xho1	Xho1	649	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.66/32.3kd	YES

2013/8/23
SHANYI

Lane	Vector Name	Name	Clone ID	Host	Induction	Purification	pI/Mw	Expression
1	pT7A3-Not1	Not1	635	5 ml, Tuner(DE3)plysN	0.2 mM IPTG 15oC o/n	Ni beads	5.64/47kD
2	pT7A3-Xba1	Xba1	636	5 ml, Tuner(DE3)plysN	0.2 mM IPTG 15oC o/n	Ni beads	5.94/28.4kd
3	pT7A3-Nco1	Nco1	637	5 ml, Tuner(DE3)plysN	0.2 mM IPTG 15oC o/n	Ni beads	5.47/36kd	yes
4	pT7A3-BglII	BglII	638	5 ml, Tuner(DE3)plysN	0.2 mM IPTG 15oC o/n	Ni beads	5.66/31kd
5	pT7A3-BamHI	BamHI	639	5 ml, Tuner(DE3)plysN	0.2 mM IPTG 15oC o/n	Ni beads	5.47/29.6kd	YES
6	pT7A3-EcoR1	EcoR1	640	5 ml, Tuner(DE3)plysN	0.2 mM IPTG 15oC o/n	Ni beads	5.96/36.5kd
7	pT7A3-EcoRV	EcoRV	641	5 ml, Tuner(DE3)plysN	0.2 mM IPTG 15oC o/n	Ni beads	8.62/32.7kd
8	PageRuler Plus protein ladder
9	pT7A3-Nhe1	Nhe1	643	5 ml, Nico21(DE3)plysN	0.2 mM IPTG 15oC o/n	Ni beads	6.06/42.7kd
10	pT7A3-SalI	SalI	644	5 ml, Tuner(DE3)plysN	0.2mM IPTG 15oC o/n	Ni beads	6.01/31kd
11	pT7A3-PstI	PstI	645	5 ml, Tuner(DE3)plysN	0.2 mM IPTG 15oC o/n	Ni beads	7.32/41kd	YES
12	pT7A3-Xho1	Xho1	649	5 ml, Tuner(DE3)plysN	0.2 mM IPTG 15oC o/n	Ni beads	8.66/32.3kd	YES