636 637 638 639 640 641 643 644 645 649 1844

2013-9-23

The following was done by Yingying

Lane	Name	Clone ID	Host	Induction	Purification	pI/Mw	Expression
1	pT7A3-Xba1	636	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.94/28.4kd
2	pT7A3-Nco1	637	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.47/36kd	YES
3	pT7A3-BglII	638	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.66/31kd
4	pT7A3-BamHI	639	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.47/29.6kd	YES
5	pT7A3-EcoR1	640	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.96/36.5kd
6	pT7A3-EcoRV	641	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	8.62/32.7kd
7	pT7A3-Nhe1	643	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	6.06/42.7kd
8	pT7A3-SalI	644	5 ml, Nico21(DE3)plysH	0.2mM IPTG RT o/n	Ni beads	6.01/31kd
9	pT7A3-PstI	645	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	7.32/41kd
10	pT7A3-Xho1	649	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	8.66/32.3kd	YES
11	pT7A3-His-MBP-(E3C)-FluA-NP	1844	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.82 / 100960.14	YES

9/28/2013

The following was done by Shanyi

Lane	Name	Clone ID	Host	Induction	Purification	pI/Mw	Expression
1	His-MBP-TaqMuts, elute from amylose beads				purified Ni then MBP
2	His-MBP-TaqMuts amylose beads Flow through				purified Ni then MBP
3	Fermentas PageRuler Plus
4	pT7A3-Xba1	636	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.94/28.4kd	yes
5	pT7A3-Nco1	637	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.47/36kd	YES
6	pT7A3-BglII	638	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.66/31kd	yes
7	pT7A3-BamHI	639	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.47/29.6kd	YES, smear
8	pT7A3-EcoR1	640	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.96/36.5kd	no
9	pT7A3-EcoRV	641	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	8.62/32.7kd	yes
10	pT7A3-Nhe1	643	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	6.06/42.7kd	?
11	pT7A3-SalI	644	5 ml, Nico21(DE3)plysH	0.2mM IPTG RT o/n	Ni beads	6.01/31kd	yes
12	pT7A3-PstI	645	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	7.32/41kd	no
13	pT7A3-Xho1	649	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	8.66/32.3kd	no
14