654 655 1801 1816 1821 1829 1830 1831 1823 1824 1825

Protein Expression

2013/8/23
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw
1	pT7A3-Monellin-E	Monellin-E	654	5 ml, Tuner(DE3)plysN	0.2 mM IPTGRT o/n	Ni beads	8.0/11.4kd
2	pT7A3-Monellin-Y	Monellin-Y	655	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.68/11.2kd
3	pT7A30-KOD-sso7d	KOD-sso7d	1801	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.28/102kd
4	pCold-MBP-(E3C)-Enterokinase	Enterokinase	1816	5 ml, C3030 SHUFFLE T7 express lysy	0.2 mM IPTG 15oC o/n	Ni beads	5.90/27kd
5	pT7A30-TthPol-C6XHis	TthPol	1821	5 ml, Tuner(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	98.7KD
6	pCold-FN3K	FN3K	1829	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG 15oC o/n	Ni beads	7.29 / 38063.47
7	pET28b-FN3K	FN3K	1830	5 ml, C3013 T7express LysY	0.2 mM IPTG RT o/n	Ni beads	7.29 / 38794.21
8	pET30b-FN3K	KOD-sso7d	1831	5 ml, C3013 T7express LysY	0.2 mM IPTG RT o/n	Ni beads	6.95 / 36349.49
9	PageRuler Plus Prestained protein ladder
10	pT7A3-MBP-(E3C)-FN3K	FN3K	1822	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	80KD
11	pCold-MBP-(a3C)-NRF2-3C	NRF2	1824	5 ml, BL21DE3pRare2pLysS	0.2mM IPTG RT o/n	Ni beads	72kd
12	pCold-MBP-(a3C)-NRF2-pLacZ-3C	NRF2	1825	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	72kd