683 pLysN-mKpn1

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Cloning of 683 pLysN-mKpn1

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Purpose: Cloning of 1683 pLysN-mKpn1

Date: 2013-10-21---

Notebook:0

Page:

Clone ID:683

Experimenters:  shanyi

Results

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PCR

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PCR using pUS40-mKpn1 as template
mKpn1-Nde1-F ctagca catatggataatcatgctaacga
mKpn1-Xho1-R gtcgagctcgagctatattatttctatttttttata

digest with NdeI+XhoI



Colony Screening

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Date: 2013-10-23

Make miniprep, digest with NdeI+XhoI

SENT 683-8 TO SEQUENCE



Result intepretation/Summary

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Conclusion

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