715-pT7A3E-wt-Xho1
Cloning of 715-pT7A3E-wt-Xho1
[edit]Purpose: Cloning of 715-pT7A3E-wt-Xho1
Date: 2013-12-6---2013-12017
Notebook:0
Page:
Clone ID:715
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR from genomic DNA
Xho1-Nde1-F
Xho1-Xho1-R
| lane |
1 |
2 |
| PCR |
1892 pT7A3E | |
| Nde1 +Xho1 | ||
Colony Screening
[edit]Date: 2013-12-17
Make miniprep and digest with Nde1 +Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]
Fix of Cloning of 715-pT7A3E-wt-Xho1
[edit]Purpose: Cloning of 715-pT7A3E-wt-Xho1
Date: 2014--1-9---2014-1-14
Notebook:0
Page:
Clone ID:715
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:F primer+ Xho-fix-R
pcr2:xho-fix-F+ R primer
| lane |
1 |
2 |
| PCR |
1892 pT7A3E | |
| Nde1 +Xho1 | ||
ligate and transform in 1878pLysN-Plac-mXho1 competent cell ,grow in k/chl plate
Colony Screening
[edit]Date: 2014-1-13
Make miniprep and digest with Nde1 +Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]
Fix of Cloning of 715-pT7A3E-wt-Xho1
[edit]Purpose: Cloning of 715-pT7A3E-wt-Xho1
Date: 2014--1-9---2014-1-14
Notebook:0
Page:
Clone ID:715
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:F primer+ Xho-fix-R
pcr2:xho-fix-F+ R primer
| lane |
1 |
2 |
| PCR |
1892 pT7A3E | |
| Nde1 +Xho1 | ||
Colony Screening
[edit]Date: 2014-1-23
Make miniprep and digest with Nde1 +Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]