726-pBAD-sfGFP-C66A
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Cloning of 726-pBAD-sfGFP-C66A
[edit]Purpose: Cloning of 726-pBAD-sfGFP-C66A
Date: 2014-1--30---2014-2-3
Notebook:0
Page:
Clone ID:726
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 546 pBad24-sfGFP as template
new oligo
sfGFP-C66A-F
old oligo
Ecoli-sfGFP20
Gel Purify PCR product,
then use the purified PCR fragment as template, run PCR
Ecoli-sfGFP1
Ecoli-sfGFP20
| lane |
1 |
2 |
| PCR product |
pBad24 545 | |
| EcoR1 + HindIII | ||
Colony Screening
[edit]Date: 2014-2-3
Make miniprep and digest with EcoR1 + HindIII
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]