732-pBad24-SuperNova
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Cloning of 732-pBad24-SuperNova
[edit]Purpose: Cloning of 732-pBad24-SuperNova
Date: 2014-4-4---2014-4-9
Notebook:0
Page:
Clone ID:732
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:Supernova-1-1to Supernova-1-10
pcr2:Supernova-2-1 Supernova-2-10
PCR:pcr1+pcr2
| Lane |
1 |
2 |
| PCR |
545 pBad24 | |
| EcoR1 + HindIII | ||
Colony Screening
[edit]Date: 2014-4-9
Make miniprep and digest with EcoR1 + HindIII
