735 pHCMC04-Enterokinase
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Cloning of 735 pHCMC04-Enterokinase
[edit]Purpose: Cloning of735 pHCMC04-Enterokinase==
Date: 2014-7----
Notebook:0
Page:
Clone ID:735
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 1731 1 ul of 10 uM enterokinase-BamH1-F1 1 ul of 1 uM enterokinase-BamH1-F2 1 ul of 10 uM enterokinase-Xma1-R
digest with BamH1+ Xma1, clone to 2271 pHCMC04
Colony Screening
[edit]Date: 2014_2_14
Make miniprep, use EcoRV to find positive clone
use BamH1+ Xma1 to verify
735-1 missing one G, need to fix
CLONING
[edit]
PCR from735-1
1 ul of 10 uM enterokinase-BamH1-F1
1 ul of 10 uM enterokinase-Xma1-R
digest with BamH1+ Xma1, clone to 2271 pHCMC04
Colony Screening
[edit]Date: 2014_2_14
Make miniprep, use EcoRV to find positive clone
