757 1139 1577 2005 2361 2384 2385 2388 2389 2390 2392

Protein Expression

2015/1/7
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	SAMPLE	pI/Mw	express
1	pEB3-Nco1	Nco1	757	15 ml, Nico21(DE3)plysN	0.2%Arabinose RT O/N	s	5.8/32.4	/??
2	p8H-EcoSSB		1139	15 ml, Nico21(DE3)plysN	0.5mM IPTG RT O/N	s	6.55/21.5	yes
3	pT7A3E-RNase-R		1577	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	S	8.71/93.8	yes
4	pT7A3E-HC-RNAse1		2005	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N		8.57/31.6	yes
5	pageRuler Plus Prestained protein ladder
6	pEB3-T4-uvsY		2361	15 ml, Nico21(DE3)plysN	0.2%Arabinose RT O/N	s	7.85/17.5	no
7	pT7A3E -Apyrase		2384	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	s	7.01/49.2	yes
8	pL6H-TRX-Tat-NLS-iFlp-6H		2385	15 ml, Nico21(DE3)plysN	42oc O/N	s	9.32/66.1	no
9	pEt30b-Gst-PcrA		2388	15 ml, Nico21(DE3)plysN	0.5mM IPTG RT O/N	s	5.82/83.2	yes
10	pET30B-HCMVUL98-		2389	15 ml, Nico21(DE3)plysN	0.5mM IPTG RT O/N	S	6.28/38.7	---
11	pT7A3-Gst-MutL		2390	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	S	5.83/70.4	yes
12	pEB3-uvsX		2392	15 ml, Nico21(DE3)plysN		s	5.62/45.1	yes

Lane	Name	Protein Name	Clone ID	Host	Induction	SAMPLE	pI/Mw	express
1	pEB3-Nco1	Nco1	757	15 ml, Nico21(DE3)plysN	0.2%Arabinose RT O/N	p	5.8/32.4	/??
2	p8H-EcoSSB		1139	15 ml, Nico21(DE3)plysN	0.5mM IPTG RT O/N	p	6.55/21.5	yes
3	pT7A3E-RNase-R		1577	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p	8.71/93.8	NO
4	pageRuler Plus Prestained protein ladder
5	pT7A3E-HC-RNAse1		2005	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N		8.57/31.6	yes
6	pEB3-T4-uvsY		2361	15 ml, Tuner(DE3)plysN	0.2%Arabinose RT O/N	p	7.85/17.5	no
7	pT7A3E -Apyrase		2384	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p	7.01/49.2	yes
8	pL6H-TRX-Tat-NLS-iFlp-6H		2385	15 ml, Nico21(DE3)plysN	42oc O/N	p	9.32/66.1	YES
9	pEt30b-Gst-PcrA		2388	15 ml, Nico21(DE3)plysN	0.5mM IPTG RT O/N	p	5.82/83.2	NO
10	pET30B-HCMVUL98-		2389	15 ml, Nico21(DE3)plysN	0.5mM IPTG RT O/N	p	6.28/38.7	---
11	pT7A3-Gst-MutL		2390	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p	5.83/70.4	yes
12	pEB3-uvsX		2392	15 ml, Nico21(DE3)plysN		p	5.62/45.1	yes