757 2325 2326 2361 2381 2385 2389 2394 2399 2400
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Protein Expression
[edit]2015/1/19
SHANYI
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pEB3-Nco1 |
Nco1 |
757 |
15 ml, Tuner(DE3)plysN |
0.2%Arabinose RT O/N |
s |
5.8/32.4 |
no |
| 2 |
pET30b-Ecoli-MutL |
2325 |
15 ml,
Nico21(DE3)plysN |
0.5mM IPTG RT O/N |
s |
6.38/69 |
no | |
| 3 |
pET30b-Ecoli-MutH |
2326 |
15 ml,
Nico21(DE3)plysN |
0.5mM IPTG RT O/N |
S |
6.56/26.6 |
yes | |
| 4 |
pEB3-T4-uvsY |
2361 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
17.5 |
no | ||
| 5 |
pageRuler Plus Prestained protein ladder |
|
||||||
| 6 |
pT7A3-TRX-Tat-NLS-iFlp-6H |
|
15 ml,
Nico21(DE3)plysN |
0.1mM IPTG RT O/N | s |
9.32/66.1 |
no | |
| 7 |
pL6H-TRX-Tat-NLS-iFlp-6H |
2385 |
15 ml,
Nico21(DE3)plysN |
42oc O/N |
s |
9.32/66.1 |
no | |
| 8 |
pET30b-HCMVUL98 |
2389-1 |
15 ml,
Tuner(DE3)plysN |
0.5mM IPTG RT O/N |
s |
6.28/29 |
no | |
| 9 |
pET30b-HCMVUL98 |
2389-2 |
15 ml, Tuner(DE3)plysN |
0.5mM IPTG RT O/N |
s |
6.28/29 |
no | |
| 10 |
pT7A30-RecO |
2394 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
S |
9.66/29 |
no | |
| 11 |
pET28b-scRAD52 |
2399 |
15 ml,
Nico21(DE3)plysN |
0.5mM IPTG RT O/N |
S |
5.6/44 |
yes | |
| 12 |
pET30b-hRAD51 |
2400 |
15 ml,
Nico21(DE3)plysN
|
0.5mM IPTG RT O/N |
s |
5.34/37.2 |
yes |
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pEB3-Nco1 |
Nco1 |
757 |
15 ml, Tuner(DE3)plysN |
0.2%Arabinose RT O/N |
p |
5.8/32.4 |
no |
| 2 |
pET30b-Ecoli-MutL |
2325 |
15 ml,
Nico21(DE3)plysN |
0.5mM IPTG RT O/N |
p |
6.38/69 |
no | |
| 3 |
pET30b-Ecoli-MutH |
2326 |
15 ml,
Nico21(DE3)plysN |
0.5mM IPTG RT O/N |
p |
6.56/26.6 |
yes | |
| 5 |
pEB3-T4-uvsY |
2361 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
p |
17.5 |
no | |
| 4 |
pageRuler Plus Prestained protein ladder |
|
||||||
| 6 |
pT7A3-TRX-Tat-NLS-iFlp-6H |
|
15 ml,
Nico21(DE3)plysN |
0.1mM IPTG RT O/N | p |
9.32/66.1 |
yes | |
| 7 |
pL6H-TRX-Tat-NLS-iFlp-6H |
2385 |
15 ml,
Nico21(DE3)plysN |
42oc O/N |
p |
9.32/66.1 |
yes | |
| 8 |
pET30b-HCMVUL98 |
2389-1 |
15 ml,
Tuner(DE3)plysN |
0.5mM IPTG RT O/N |
p |
6.28/29 |
no | |
| 9 |
pET30b-HCMVUL98 |
2389-2 |
15 ml, Tuner(DE3)plysN |
0.5mM IPTG RT O/N |
p |
6.28/29 |
no | |
| 10 |
pT7A30-RecO |
2394 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
p |
9.66/29 |
no | |
| 11 |
pET28b-scRAD52 |
2399 |
15 ml,
Nico21(DE3)plysN |
0.5mM IPTG RT O/N |
p |
5.6/44 |
yes | |
| 12 |
pET30b-hRAD51 |
2400 |
15 ml,
Nico21(DE3)plysN
|
0.5mM IPTG RT O/N |
p |
5.34/37.2 |
yes |