940 pET28b-yPolyA-Po
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938 digestion with Nde1-Xho1, Fastdigest Buffer
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8,9 | 10,11 | 13 |
| 938 PNGase_F | 939 Tte-ExoIII |
942 VVN |
3122 CRM197 |
931, 1, PAK16D ligase |
931, 2, PAK16D ligase |
1855 yPolyA-Pol |
1540 | 2046 | 2046 |
| Nde1-Xho1 | Nde1-Xho1 |
Nco1-Xho1 |
Nde1-Xho1 |
Nde1-HindIII |
HindIII-Xho1 | Nde1-Xho1 | Nde1-Xho1 | Nde1-Xho1 | Nco1-Xho1 |
| pJet1.2 | pJet1.2 |
pJet1.2 |
pJet1.2 |
pJet1.2 |
pJet1.2 |
pEC2- |
pET30b | pET28b | pET28b |
| 1145 |
780 |
716 |
1798 |
1174 |
986 |
1713 | there is no Xho1 site | 1377 | ~1377 |
940 in pET28b
1/29/18
