985 pCold-GST-3C-AP205-M13-g3p
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985 subclone
Vector:1987 pT7A3 digested with Nde1-Xho1
Insert:983(973-2 pMiniT2) digested with Nde1+Sal1
Size:500 bp
Date:4/11/2018
[edit]Miniprep digestion of 3152_985
| # | Lane | Size | Enzymes | Note |
| 3152 | 1-4 | 371bp | BsrG1-Nhe1 |
size is the same as digested vector,need to do sequencing |
| 985 | 5-8, Lane 9 is +control | 402bp | Nde1+Kpn1 | good |
