994 pT7A3-GST-CD133-EC2
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Vector digestion
PCR of 994_995
Miniprep digestion of 998_1000_994_995'
| # | Lane | Enzymes | Size | Note |
| 998 | 1-4 | HindIII+Xho1 | ||
| 1000 | 5-8 | HIndIII+Xho1 | ||
| 994 | 9-12 | BamH1+Xho1 | 616 | |
| 995 | 13-16 | BamH1+Xho1 |
994: no good
Date:6/18/2018
[edit]Vector digestion
Cloning of
[edit]Purpose: Cloning of 994 pCold-GST-CD133-EC2
Date: 06, 29, 2018
Notebook:
Page:
Clone ID: 994
Experimenters: yijia
Results
[edit]Cloning
[edit]PCR from 3147 pANT7_cGST-PROM1
PCR994
.JPG)
'Vector is 3225pT7A3-GST-MICA 'digestion
Colony screen
[edit]Miniprep of 129_994_1000_3206
| # | Vector | Lane | Enzymes | Size | Clone# | Note |
| 129 | 1801 | 1-4 | Nde1+HindIII | 2433 | 2,4 look good | 129-2;129-4 |
| 994 | 3225 | 5-8 | BamH1+Xho1 | 609 | 5,6,look good | Vecotor doesn't have BamH1 Site,994-1;994-2 |
| 1000 | 1708 | 9-12 | HIndIII+Xho1 | 118 | 11,12 looks good | 1000-3;1000-4 |
| 3206 | 3165 | 13-16 | HIndIII+BamH1 | 1460 | need to do PCR screening |
Sequencing: 994 #1 missing 1 T, need to screen plasmids using Nde1 -Xho1
Miniprep digestion of 994
