995 pCold-GST-CD133-EC3

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Vector digestion

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PCR of 994_995

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Miniprep digestion of 998_1000_994_995'

# Lane Enzymes Size Note
998 1-4 HindIII+Xho1

1000 5-8 HIndIII+Xho1

994 9-12 BamH1+Xho1

995 13-16 BamH1+Xho1 528

RTENOTITLE995: no good

Date:6/5/2018

[edit]

Miniprep of 3191_984_995

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Miniprep digestion of 3218_995_3197

Vector is 3225

RTENOTITLE sequencing: 995 #1 is junk

995PCR

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Miniprep digestion of 931-1_995

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Redo

[edit]

Date:7/10/2018

995 PCR

Template:3147

Size:615bp

Size: RTENOTITLE

995 PCR screening