999 pT7A30-SssI
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Cloning of SssI into pT7A30
[edit]Purpose:
Date: 07/09/2018
Notebook:
Page:
Clone ID: 999
Experimenters: yijia
Results
[edit]Subcloning
[edit]Date: 7/ 6 /2018
By:
parent (insert):1916pMiniT2
vector:1873
Gel:
Ligation: 30 min with T4 DNA ligase
Competent cells: NEBTurbo
redo 2nd time
[edit]999 subclone
Restriction Digestion
Date: 7/13/2018
By: Yijia
miniprep digestion
miniprep digestion 3225_999_931
| # | Lane | Vector | Enyzmes | Size | note |
| 3225 | 1-4; 5 is + control | 1851 pT7A3-GST | Nhe1+Xho1 | 383bp | |
| 999 | 6-9;10 is +control | 1873 | Nde1+Xho1 | 1182bp | |
| 931 | 11-14;15 is + control | 931-1 | HindIII+Xho1 | failed |
