Baculovirus Cre FLP QC 20141020 20141025
VLP from sf9 cells.
Original FLP: VLP in sf9 cell culture medium
Final Purified FLP: Concentrated from 1L to to 200ml (FLP) by ultrafiltration, purified by gel filtration, then ultrafiltrated again, filtered on 10/10/2014
Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration , filtered on 10/08/2014
Final Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration ,then ultrafiltrated again, filtered on 10/10/2014
Reporter: 2013 for Flp, 2181 for Cre
Oct. 20 2014 Morning : split 293 cells
Oct. 20 2014 afternoon Transfect 293 cell (70--80%) using lipofectamin 2000
Oct.21 2014 10:00 am: 70%-80% cells turns red , cells are in good condition, add Flp and Cre to reporter cells
|
A1: 0ul original FLP |
A2: 50ul original FLP |
A3: 100 ul original FLP |
A4: 0 ul Purified cre |
A5: 25ul Purified cre |
A6: 75ul Final Purified FLP |
| B1:
50ul Final Purified FLP (filtered on 10/10/2014) |
B2:
100ul Final Purified FLP (filtered on 10/10/2014) |
B3:
150ul Final Purified FLP (filtered on 10/10/2014) |
B4:
25 ul Final Purified FLP (filtered on 10/10/2014) |
B5:
50 ul Final Purified FLP (filtered on 10/10/2014) |
B6:
75 ul Final Purified FLP (filtered on 10/10/2014) |
|
C1: 50ul Final Purified FLP (filtered on 10/12/2014) |
C2:
100ul Final Purified FLP (filtered on 10/12/2014) |
C3:
150ul Final Purified FLP (filtered on 10/12/2014) |
C4:
25ul Final Purified Cre (filtered on 10/12/2014) |
C5:
50ul Final Purified Cre (filtered on 10/12/2014) |
C6:
75ul Final Purified Cre (filtered on 10/12/2014) |
Oct.22 2014 24hours 
Oct.23 2014 48hours 
Oct242014
