Cre 1708 2323 2337 2349 2360 2361 2362 2363

Protein Expression

2014/10/22
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw	express
1	pET28b-HTNCre		375	8L Nico21(DE3)plysN	0.2 mM IPTG  RT O/N	Ni --sp--ni
2	pT7A3-HTNCre		1708	15 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads
3	2323 pR-Enterokinase		2323-5	15 ml, Ecoli K12JM101	37oC O/N	Ni beads	29.6
4	2323 pR-Enterokinase		2323-1	15 ml, Ecoli K12JM101	37oC O/N	Ni beads	29.6
5	PageRuler Plus Prestained protein ladder
6	pET28b-Cas9-C6H		2337	15 ml, Nico21(DE3)plysN	0.4 mM IPTG RT O/N	Ni beads	160.2
7	pT7A3-Cas9-Nickas		2349	15 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	160.2
8	pET30b-T4-uvsY		2360	15 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	17.5
9	pEB3-T4-uvsY		2361	15 ml, Nico21(DE3)plysN	0.2 % Arabinose RT O/N	Ni beads	17.5
10	pT7A3-MBP-(Tev)-HCMVUL98		2362	15 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	110
11	pT7A3-MBP-(Tev)-HCMVUL98		2362	15 ml, Tuner(DE3)plysN-tev	0.2 mM IPTG RT O/N	Ni beads	67+42
12	pT7A3E-T4-BGT		2363	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	40.6