Cre purification

File:Cre gel-filtration purification chromatogram.pdf

Gel filtration using HiLoad 16X60 Superdex

1X TBS, 1.5 ml/min

combine fractions 29 to 38, OD280 is 0.19

MW ladder: 250, 130, 100, 70(red), 55, 35, 25(red), 15, 10 (green)

Fraction #