Cre purification2014 10 27

2014/10/27

lane1-5 : 1L 1708 pT7A2-HTNCre Nico21(DE30plysN

                without antiform,

                when OD600>1.2, use o.5mM IPTG RT induce overnight, elute from 5ml HisTrap HP

lane6-9:  1L 1708 pT7A2-HTNCre Nico21(DE30plysN

                with antiform and 5020,

               when OD600>1.2, use o.5mM IPTG RT induce overnight, elute from 5ml HisTrap HP

lane 10: 2048 MBP-HCMVUL98(before 3c digestion)

lane 11: 2048 MBP-HCMVUL98(after 3c digestion)