Cross-linkers

Cross Linker Structure

N,N′-Bis(acryloyl)cystamine

DSP: http://www.proteochem.com/dspcrosslinker1gram-p-97.html

dimethyl 3,3 -dithiobispropionimidate (DTBP)

bis(2-methacryl-oyloxyethyl) disulfide

Conjugation of DSP with PEI

CBA, cysteamine bisacrylamide

N,NŽ-bis-Acrylylcystamine; N,NŽ-bis(Acryloyl)cystamine; bis-Acrylylcystamine: BAC

1. Dissolve 22.5 g cystamine dihydrochloride (0.1 mol)   in 100 ml water in a three-necked flask.

2. Cool solution to 0 °C.

3. Add acryloyl chloride dichloromethane solution (0.2 mol, 20 ml) and aqueous NaOH solution (0.2 mol, 10 ml) simultaneously.

4. Stir reaction mixture continuously at room temperature for 16 h.

5. Filter the raw product, and wash three times with water.

6. Crystallization using ethyl acetate.

7. Remove the solvent under vacuum.

dithiobis(succinimidylpropionate) (DSP)

DSP and DTSSP are moisture-sensitive. Store desiccated at 4-8°C. To avoid moisture condensation onto the product, vial must be equilibrated to room temperature before opening (equilibration may require 30 minutes).

Reconstitute these crosslinkers immediately before use. The NHS-ester moiety readily hydrolyzes and becomes non-reactive; therefore, do not prepare stock solutions for storage. Discard any unused reconstituted crosslinker.

Imidoester Crosslinker Reaction