Daniel 2017 06 27

lane 1-16 are 826 pHis-MBP-myc-His colony PCR screening, primers are T7F and 819-His-Myc-His-B, 4.5.8.11 may be positive clones.

lane 1 is 826 pHis-MBP-myc-His colony PCR screening, lane 2-4 are 827 pHis-MBP-HA-His colony PCR screening, primers are T7F and 820-His-HA-His-B, 2.3.4 may be positive clones.

digestion with Nde1+Xba1

lane 1.2.3.4 are 826 pHis-MBP-myc-His restriction enzyme digestion, lane 5.6.7 are 827 pHis-MBP-HA-His digestion