ELISA Coating Buffer
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Bicarbonate/carbonate coating buffer (100 mM)
3.03 g Na2CO3,
6.0 g NaHCO3
1000 ml distilled water
pH 9.6,
PBS:
1.16 g Na2HPO4,
0.1 g KCl,
0.1 g K3PO4,
4.0 g NaCl (500 ml distilled water) pH 7.4.
A protein (antigen or antibody) concentration of 1-10 ug/mL in coating buffer is usually sufficient.