E coli Secrection Expresion

PelB (pectate lyase B) from Erwinia carotovora MKYLLPTAAAGLLLLAAQPAMA
OmpA (outer-membrane protein A) MKKTAIAIAVALAGFATVAQA
StII (heat-stable enterotoxin 2) MKKNIAFLLASMFVFSIATNAYA
Endoxylanase from Bacillus sp. MFKFKKKFLVGLTAAFMSISMFSATASA
PhoA (alkaline phosphatase) MKQSTIALALLPLLFTPVTKA
OmpF (outer-membrane protein F) MMKRNILAVIVPALLVAGTANA
PhoE (outer-membrane pore protein E) MKKSTLALVVMGIVASASVQA
MalE (maltose-binding protein) MKIKTGARILALSALTTMMFSASALA
OmpC (outer-membrane protein C) MKVKVLSLLVPALLVAGAANA
Lpp (murein lipoprotein) MKATKLVLGAVILGSTLLAG
LamB (λ receptor protein) MMITLRKLPLAVAVAAGVMSAQAMA
OmpT (protease VII) MRAKLLGIVLTTPIAISSFA
LTB (heat-labile enterotoxin subunit B) MNKVKCYVLFTALLSSLYAHG

consensus signal peptide (CSP) 
atgaaaaaaaaattattggcgttagccttgttagcgttattgtttaacggcgcgcaggccatg
M  K  K  K  L  L  A  L  A  L  L  A  L  L  F  N  G  A  Q  A  M  

based on 
AACAGAAACAATAATAATGGAGTCATGAACAT 
ATGAAAAAAAAATTATTGGCGTTAGCCTTGTTAGCGTTATTGTTTAACGGCGCGCAGGCCATGGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
M  K  K  K  L  L  A  L  A  L  L  A  L  L  F  N  G  A  Q  A  M  A  H  P  E  T  L  V  K  V  K  D  A  

mutant S48 
atgaaaagaaaattattggtattagccctgttagcggtactgttcaacggcgagcatgccatg
M  K  R  K  L  L  V  L  A  L  L  A  V  L  F  N  G  E  H  A  M
MKRKLLVLALLAVLFNGEHAM 
..R...V.....V....EH..