Enzymes Requested by Customers

Name	Clone ID	Cat #	Competitor	Cat #, Size & Price	Customer	Note	Status
Exonuclease I	1570  pT7A3-MBP-(3C)-Exo-1		Thermofisher	EN0582， 20,000 units, $338.00	王敏，上海， 1000 KUnits, total: $16,900	Description Exonuclease I (Exo I) degrades single-stranded DNA in a 3'5' direction, releasing deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaving 5'-terminal dinucleotides intact. It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups (1). Applications  Primer removal from PCR mixtures:  prior to PCR product sequencing (2),  for one-tube “megaprimer” PCR mutagenesis (3).  Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures.  Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus (4). Source E.coli cells with a cloned E.coli sbcB gene.
FastAP Thermosensitive Alkaline Phosphatase				EF0651 ,1,000 units, $73.25	王敏，上海， 200 KUnits, total: $14650	Description Thermo Scientific™ FastAP™ Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'- phosphate groups from DNA, RNA and nucleotides. This enzyme also removes phosphate groups from proteins. FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends in 10 min at 37 °C. The enzyme is inactivated in 5 min at 75 °C. Therefore, removal of alkaline phosphatase is not required prior to ligation. Applications  Dephosphorylation of cloning vector DNA to prevent recircularization during ligation.  Simultaneous digestion and dephosphorylation of vector DNA.  PCR product clean-up: nucleotide degradation prior to sequencing of PCR product.  Dephosphorylation of nucleic acid 5’-termini prior to labeling with T4 Polynucleotide Kinase.  Other applications where dephosphorylation of DNA and RNA substrates is necessary.  Protein dephosphorylation. Source E.coli cells with a cloned bacterial AP gene.
VVTopo	824 pET28b-VVTopo				order # 4988		sequenced
VVN endonuclease	942				Tien Gen	for making NGS library
T7 endonuclease 1	941				Tien Gen	for making NGS library
T4 DNA polymerase					XIaoli
T4 DNA ligase					XIaoli
PAK16D ligase	931				for a biotech company in Shanghai
PolyA polymerase, E coli 940 pET28b-yPolyA-Pol	940	EG-150			王敏 Boston Children's hospital
pET28b-Cid1, yeast polyU	927	EG-151			Boston Children's hospital	yeast polyU polymerase, http://www.excellgen.net/eln/index.php/927_Cid1
PolyA polymerase, Yeast					王敏
M.sssl	1916				Zhao Fangping	Thermo EM0821
T4 PNK					Zhao Fangping	Invitrogen: VGN2304: T4 PNK kit
Thermosensitive Alkaline Phosphatase.					Zhao Fangping	Invitrogen: EF0654 FastAP Thermosensitive Alkaline Phosphatase.
T5 exonuclease pET8XHis-T5Exo	1021	EG-166			胡亚东（分子原料线PM） <1833405799@qq.com>	use 2434-pCold-MBP-(Ent-TEV)-T5-Exo, or 1567 pT7A3-T5-Exo for expression