Expression Optimization of Cre Recombinase

5/20/2019, by Paul XU

Medium	5020	Antiform	Indution	Cell Wet Weight, g	Cre
Super Broth, 1 L			0.8 mM IPTG, 37 oc 2 hours	10.9
Super Broth, 1 L		1 drop	0.8 mM IPTG, 37 oc 2 hours	7.6
High Yield LB, 1 L			0.8 mM IPTG, 37 oc 2 hours	7.2
High Yield LB, 1 L	20 ml		0.8 mM IPTG, 37 oc 2 hours	9.5
High Yield LB, 1 L	20 ml	1 drop	0.8 mM IPTG, 37 oc 2 hours	5.6

IPTG from GoldBio

Conclusion: Do not use antiform.