FLP Cre VLP Activity assay

Making VLP

2119 pCMV5-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP

2138 pCMV5-pene-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP

2136 pCMV5-tat-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP

2137 pCMV5-CD63-Flpe + /- 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP

2140 pCMV5-tat-CD63-Flpe + /- 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP

2139 pCMV5-pene-CD63-Flpe + /- 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP

2128 pCMV5-Mfge8-Tat-TagGFP-MFGE8

2123 pCMV5-Mfge8-Tat-Cre-MFGE8 + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP

2122 pCMV5-Mfge8-Tat-FLP-MFGE8 + /- 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP

Activity assay

add 0.5 ml condition culture medium to 24 well plate, 293T cells transfected with 2013 pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP or 1522 CMV-Brainbow-2.1-R

3 days after transfection

7days after transfection