Flp VLP Optimization

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FRT Reporter Assay using FLP plasmid

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1. Reporter: 1444 pLVX-Kozak-FRT-TurboRFP-ODC-FRT-TurboGFP

FLP:

471 pAAV.CMV-FLPe

1526 pCDNA3.1-NLS-FLPo

1525 pCDNA3.1-FLPe

1520 pDIRE

1445 pCMV5-Gag-NLS-Flp-WPRE-oriP

1857 pOG44

VLP from 1445 pCMV5-Gag-NLS-Flp-WPRE-oriP


2. Reporter: 1922 pLVX-Kozak-FRT-TurboRFP-ODC-2Stop-FRT-TurboGFP


FLP:

471 pAAV.CMV-FLPe

1526 pCDNA3.1-NLS-FLPo

1525 pCDNA3.1-FLPe

1520 pDIRE

1445 pCMV5-Gag-NLS-Flp-WPRE-oriP

1857 pOG44

VLP from 1445 pCMV5-Gag-NLS-Flp-WPRE-oriP


VLP Titering

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