Flpy

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Protein Expression

[edit]

2013/12/5
SHANYI


Lane
Name
Protein Name
Clone ID
Host
Induction
Purification
pI/Mw
9
pET28b-yFLP
yFLP           

1853

5 ml,

T7 EXPRESS



0.5 mM IPTG

37oC 2h

Ni beads










1
pT7A30-yFLP

1860

5 ml,

BL21DE3pRare2pLysS

2

 5 ml,
Nico21(DE3)pRare2pLysS

3

5 ml,

Rosetta™(DE3)pLysS

4

 5 ml,
Tuner(DE3)pRare2pLysS

5

 5 ml,
Nico21(DE3)plysH

6
  5 ml,

BL21(DE3)

7
  5 ml,

Nico21(DE3)

8
  5 ml,

Tuner(DE3)